The Interactive Effect between Kitasamycin Tartrate and Doxycycline Hydrate for Use in the Therapy and Prevention of Diseases in Veterinary Medicine
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An in vitro test on the pharmacological interaction of the antibiotic combination between kitasamycin tartrate and Doxycycline hydrate was carried out. An in vitro challenge method was used for this purpose. The results were used to calculate their interaction using the graphical representation known as ISOBOLOGRAM. The pure salts of Kitasamycin tartrap and Doxycycline Hydrate were used and were obtained from the manufacturer of the product, Lapi - Doxi.
The agar bioassay method was used. The following criteria were used to quantify the results: A synergistic effect was defined when the actual/theoretical effect (SMEF) was greater than 1. The additive effect was defined when the SMEF was equal to 1. The area between the antagonism and additive effect was defined as the zone of indifference. When studying the interaction between florfenicol and tylosin tartrate it was observed that the best SMF of this antibiotic combination was 1.45 when the concentration ratio between the antibiotics was 1 : 4 (Kitasamycin tartrate and doxycycline hydrate respectively). Consequently it can be postulated that the combination of kitasamycin tartrate with doxycycline hydrate showed a synergistic effect and can be recommended in poultry and swine for the treatment of mycoplasma infections, most gram + and gram- germs and other infectious agents sensitive to the formulation.
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