Antimicrobial Potential of Some Plant Extracts against Multidrug Resistant Pathogenic Bacteria: Staphylococcus Aureus and Klebsiella Pneumonia

DATE PUBLISHED
February 19, 2019
SECTION
Articles

Abstract

Plants have been extensively studied as alternative agents for prevention and treatment of infectious diseases. The present work evaluates the inhibitory efficacy of different extracts prepared from seven plant species (Erica lusitanica, Hypericum canariensis, H. inodorum, H. perforatum, Paeonia broteri, Quercus faginea subsp. broteroi and Sanguisorba hybrida) against five standard strains and clinical isolates of human pathogenic bacteria (Staphylococcus aureus and Klebsiella pneumonia). In vitro antibacterial activity was evaluated using the serial broth microdilution method.

The polar extracts were more effective against both Gram (+) and Gram (–) strains.  The MeOH and H2O extracts of S. hybrida (leaves and stems), P. broteri (leaves) and H. perforatum (leaves and stems), showed high-moderate antibacterial activity against all the five multidrug resistant (MDR) bacteria tested. These plant species appear as potential leads against MDR bacteria.

Keywords

PLANT CRUDE EXTRACTS; ANTIBACTERIAL ACTIVITY; MICRODILUTION METHOD; MULTIRESISTANT BACTERIA; STAPHYLOCOCCUS AUREUS; KLEBSIELLA PNEUMONIAE

References

Bandeira, M., Borges, V., Gomes, J. P., Duarte, A., Jordão, L. 2017. Insights on Klebsiella pneumoniae Biofilms Assembled on Different Surfaces Using Phenotypic and Genotypic Approaches. Microorganisms. 2017 Apr 3; 5 (2). pii: E16. doi: 10.3390/microorganisms5020016.

Butler, M.S. 2004. The role of natural product chemistry in drug discovery. J. Nat. Prod. 67: 2141-2153.

Butler, M.S. 2005. Natural products to drugs: natural product derived compounds in clinical trials. Nat. Prod. Rep. 22:162-195.

Butler, M. S., Buss, A. D. 2006. Natural products - The future scaffolds for novel antibiotics? Biochem. Pharmacol. 71: 919-929.

Cazarolli, L.H., Zanatta, L., Alberton, E.H., Res, B.F., Maria, S., Folaor, P., Damazio, R.G., Pizzolat, M.,G. 2008. Flavonoids. Cellular and molecular mechanism of action in glucose homeostasis. Mini Rev. Med. Chem. 18: 1032-1038.

CLSI - Clinical Laboratory Standards Institute. 2008. M100-S18 Performance Standards for Antimicrobial Susceptibility Testing: 18th Informational Supplement. Clinical and Laboratory Standards Institute, Wayne, PA.

Coates, A., Hu, Y., Bax, R., Page, C.. 2002. The future challenges facing the development of new antimicrobial drugs. Nat. Rev. Drug Discov. 1: 895-910.

Cos, P., Vlietinck, A. J,, Berghe. D.V., Maes L. 2006. Anti-infective potential of natural products: How to develop a stronger in vitro proof-of-concept. J. Ethnopharmacol. 106: 290-302.

Cowan, M. M.1999. Plant product as antimicrobial agents. Clin. Microbiol. Rev. 12: 564-582.

Cragg, G.M., Newman, D.J. 2013. Natural products: A continuing source of novel drug leads. Biochim. Biophys. Acta1830: 3670-3695.

Ghasemzadeh A. and Ghasemzadeh N. 2011. Flavonoids and phenolic acids: Role and biochemical activity in plants and human. J Med Plant Res 5: 6697-6703.

Harborne, J. B. 1989. Recent Advances in Chemical Ecology. Nat. Prod. Rep., 6: 85-109.

Helander, I.M., Alakomi, H-L., Latva-Kala K., Mattila-Sandholm, T., Pol, I., Smid, E. J., Gorri,s L., Wright, A.. 1998. Characterization of the Action of Selected Essential Oil Components on Gram-Negative Bacteria J. Agric. Food Chem. 46: 3590-3595.

Kinghorn, A.D. 1992. Plants as sources of medicinally and pharmaceutically important compounds. In Nigg, H.N., Seigler, D. (Eds.), Phytochemical Resources for Medicine and Agriculture.

https://books.google.pt/books?id=YxT3BwAAQBAJ&pg=PP1&lpg=PP1&dq=Phytochemical+Resources+for+Medicine+and+Agriculture&source=bl&ots=gNyEHWByWm&sig=5NcsAuYFq6-eJq335mQdufWemWE&hl=pt-PT&sa=X&ved=0ahUKEwjJ1vLg6d3SAhWESBQKHfMrDsEQ6AEILjAD#v=onepage&q=Phytochemical%20Resources%20for%20Medicine%20and%20Agriculture

Lai, B., Teixeira, G., Moreira, I., Correia AI, Duarte A, Madureira AM. 2012. Evaluation of the antimicrobial activity in species of a Portuguese “Montado” ecosystem against multidrug resistant pathogens. J Med Plant Res 6: 1846-1852.

Madureira, A.M., Duarte, A., Correia, A. I., Teixeira, G. 2014. Sanguisorba hybrida: pharmacognostic studies and antimicrobial activity evaluation of crude extracts. Rev. Ciências Agrárias, 37 (4): 482-490.

Nazzaro, F., Fratianni, F., Martino, L., Coppola, R., Feo, V. 2013. Effect of Essential Oils on Pathogenic Bacteria. Pharmaceuticals, 6: 1451-1474; doi:10.3390/ph6121451

Olsen, I. 2015. Biofilm-specific antibiotic tolerance and resistance. Eur J Clin Microbiol Infect Dis. 34:877-86. doi: 10.1007/s10096-015-2323-z. Epub 2015 Jan 29.

Rodrigues, L., Duart,e A., Figueiredo, A.C., Brito, L.,Teixeira, G., Moldão, M., Monteiro, A. 2012. Chemical composition and antibacterial activity of the essential oils from the medicinal plant Mentha cervina L. grown in Portugal. Med. Chem. Res. 21: 3485-3490.

Saleem, M., Nazir, M., Ali, M.S., Hussain, H., Lee, Y.S., Riaz, N., Jabbar, A. 2010. Antimicrobial natural products: an update on future antibiotic drug candidates. Nat. Prod. Rep. 27: 238–254.

Scortichini, M., Rossi, M. P. 1991. Preliminary in vitro evaluation of the antimicrobial activity of terpenes and terpenoids towards Erwinia amylovora (Burrill) Winslow et al. J. Appl. Microbiol. 71: 109-112. doi:10.1111/j.1365-2672.1991.tb02963.x

Slama, T.. 2008. Gram-negative antibiotic resistance: there is a price to pay. Crit Care 12 (Suppl 4:S4) (doi:10.1186/cc6820)

Srinivasan, D., Nathan, S., Suresh, T., Lakshmana, P. 2001. Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. J Ethnopharmacol: 217-220.

Stavri, M., Piddock, L.J.V., Gibbons, S. 2007. Bacterial efflux pump inhibitors from natural sources. J. Antimicrob. Chemother. 59:1247-1260.

Trease and Evans. 2009. Pharmacognosy. 16th ed. Elsevier. London, p. 616.

Tsuchiya, H., Sato, M., Miyazaki, T., Fujiwara, S., Tanigaki, S., Ohyama, M., Tanaka, T. and Iinuma, M. 1996. Comparative study on the antibacterial activity of phytochemical flavanones against methicillinresistant Staphylococcus aureus. J. Ethnopharmacol. 50: 27-34.

Wagner, H., Bladt, S.. 1996. Plant Drug Analysis: A Thin Layer Chromatography Atlas, 2nd ed. Springer-Verlag, Berlin, p. 384.

Author Details

Madureira A M

  • iMed Universidade de Lisboa, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisboa, Portugal.
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Jordão L

  • Departamento de Saúde Ambiental, Unidade de Investigação e Desenvolvimento, Av Padre Cruz, 1649-016, Lisboa, Portugal
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Ferreira P

  • Departamento de Saúde Ambiental, Unidade de Investigação e Desenvolvimento, Av Padre Cruz, 1649-016, Lisboa, Portugal
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Duarte A

  • iMed Universidade de Lisboa, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisboa, Portugal
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Teixeira G

  • Center for Ecology, Evolution and Environmental Changes (CE3C), Faculdade de Ciências - C2, Univ. Lisboa, Cpo. Grande, 1749-016, Lisboa, Portugal. Faculdade de Farmácia, Univ. Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisboa, Portugal.
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