Study on Selection of Bacillus Sp. Strain Has Active Carbon Metabolism for Peat Treatment
Downloads
The objective of selecting strains of Bacillus sp. has high activity in carbon metabolism for peat treatment, firstly focusing on the ability to degrade lignin, cellulose, phosphorus and protein. From the gene pool, by surveying the microbial strains capable of degrading carbon-rich substrates including lignin, cellulose, insoluble phosphate, proteolytic, we have identified two Bacillus sp. strains contain Bacillus polyfermenticus and Bacillus subtilis strains have strong ability to metabolize carbon-rich substrates and decompose phosphorus in peat. The Bacillus polyfermenticus strain had cellulose, lignin, organophosphorus and starch-degrading ring diameters of 41, 40, 23 and 38 mm, respectively. The Bacillus subtilis strain had cellulose, lignin, organophosphorus and starch-degrading ring diameters of 40, 41, 24 and 29 mm, respectively. In addition, the content of humic acids formed after 48 hours of fermentation increased 2.5 times compared to the original without the addition of Bacillus polyfermenticus strain and increased 2.8 times compared to the original without the addition of Bacillus subtilis strain. Therefore, two strains of Bacillus sp. selected with high efficiency in producing humic acid products. At the same time, the selected microbial strains have a high biosafety level (level of 1, the safest level in the biosafety scale).
Anuphap Prachumwat, Suparat Taengchaiyaphum, Natthinee Mungkongwongsiri, Diva J. Aldama-Cano, Timothy W. Flegel and Kallya Sritunyalucksana. Journal of the World Aquaculture SocietyAcute Hepatopancreatic Necrosis Disease. Volume 50, Issue 1, Pages 5-17, September 4, 2018.
Muthuwan, V. 1998. A green water recirculation system for intensive culture of marine shrimp. PhD thesis, AIT, Bangkok, Thailand
Fukumori, F., T.Kudo and K Horikoshi (1985), “ J.Gen. Microbiol” , 131, 3339-3345
Bulla J.A, Costilow R, Sappe E.S (1978), “Biology of Bacillus popippiae”, Adv. Appl. Microbiol. 23: 1-18.
Logan NA, De Vos P (2015). "Bacillus". In Whitman WB (ed.). Bergey's Manual of Systematics of Archaea and Bacteria. John Wiley & Sons.
Richard J. Roberts, Gary A. Wilson & Frank E. Young (1977), “Recognition sequence of specific endonuclease Bam HI from B. amyloliquefaceiens H”, Nature 256, pp.82-84
Shinke, R., H. Nishira, and N. Mugibayyashi (1974), “Isolation of β –amylase producing microorganisms”, Agr. Biol. Chem, 38:665-666
Nguyen Huu Chan (1996), Enzymes and biocatalysts, Medicine Publishing House, Hanoi
Nguyen Huu Chan (1996), Enzymes and biocatalysts, Medicine Publishing House, Hanoi
Dong Thi Thanh Thu (1998), Basic Biochemistry Textbook, Bookcase University of Science and Technology - National University of Ho Chi Minh City
Dang Thi Mai Phuong (2010) Research on the synthesis of pectinase induction in some Bacillus strains, Master thesis in Biology, University of Education, Ho Chi Minh City. HCM, pp.24-33
Dang Thi Mai Phuong (2010) Research on the synthesis of pectinase induction in some Bacillus strains, Master thesis in Biology, University of Education, Ho Chi Minh City. HCM, pp.24-33
All Content should be original and unpublished.
